Genome-Wide Identification of the RsSWEET Gene Family and Functional Analysis of RsSWEET17 in Root Growth and Development in Radish

SWEET (Sugars Will Eventually be Exported Transporter) genes play essential roles in various biological processes, including phloem loading, sugar efflux, plant development and stress response. In this study, a total of 33 RsSWEET gene members were identified the radish genome. They could divided into four subfamilies are distributed on eight chromosomes. Cis-acting regulatory element analysis indicated that these potentially involved growth response process, circadian control light responses to numerous stresses, low-temperature drought stress. Transcriptome data revealed number exhibited specific expression patterns different tissues developmental stages radish. Moreover, several (e.g., RsSWEET2a, RsSWEET3a, RsSWEET16b RsSWEET17) showed differential profiles under abiotic cold, heat, salt, Cd Pb Remarkably, RsSWEET17 was specifically expressed cambium heterologously yeast strain EBY.VW4000, which suggested it has ability transport sugar. Notably, RsSWEET17-overexpressing Arabidopsis plants excessive root length, greater fresh weight higher soluble content (SSC) accumulation compared with wild-type (WT) plants, indicating might positively regulate taproot by strategically manipulating accumulation. Collectively, results clarify molecular mechanisms underlying RsSWEET-mediated